Physical Specifications

• Height: 22.5 in. (57 cm)
• Depth: 23.5 in. (60 cm)
• Width: 23 in. (58 cm)
• Weight: 136 lb. (62 kg)
• Wetted Surface Materials: 316 stainless steel, UHMWPE, sapphire, ruby, Tefzel® (ETFE), Teflon® (FEP and PTFE), PEEK, Fluoroloy G, Fluoroloy–08R

Environmental Specifications

• Operating Temperature 4 to 40° C
• Relative Humidity 20 to 80%, noncondensing
• Acoustic Noise <65 dB(A)

Solvent Management System Specifications

• Number of Solvents: One to four, three with vacuum degas
• Solvent Conditioning: Vacuum degas, three solvents plus purge solvent
• Typical Operating Flow Rate Range: 0.050 to 5.000 mL/min in 0.001-mL/min increments
• Programmable Flow Rate Range: 0.000 and 0.010 to 10.000 mL/min in 0.001-mL/min increments
• Compressibility Compensation: Automatic and continuous
• System Delay Volume: <400 µL, independent of backpressure, 1 mL/min
• Plunger Seal Wash: Integral, active, programmable
• Gradient Profiles: Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)]
• Dry Prime/Wet Prime: Automatic, front panel control
• Flow Ramping: Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate
• Maximum Operating Pressure: 5000 psi (345 bar) at 0.010 to 3.000 mL/min; Programmable upper and lower limits
• Composition Range: 0.0 to 100.0% in 0.1% increments
• Composition Accuracy: ±0.5% absolute, independent of backpressure (Proportioning Valve Pair Test [degassed methanol: methanol/propylparaben, 2 mL/min, 257 nm])
• A Composition Precision: <=0.15% RSD o r<=0.02 min SD, whichever is greater, based on retention time. (Degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, phenone mix, 257 nm)
• Flow Precision: <=0.075% RSD or <=0.02 min SD, whichever is greater, based on retention time (N = 6) or volumetric measures (0.200 to 5.000 mL/min), isocratic premix
• Flow Accuracy: <=1% or 10 µL/min, whichever is greater, (0.200 to 5.000 mL/ min), degassed methanol, at 600 psi backpressure

Sample Management System Specifications

• Number of Sample Plates: Total of four plates:
  - Microtiter plate – 96- or 384-well
  - Vial plate – 2.0-mL vial (48)
  - Tube plate – 0.5-mL microcentrifuge tube (48) or 1.5-mL microcentrifuge tube (24)
  - Open access plate – 2.0-mL vial (24 or 48)
• Sample Temperature Control: 4 to 40° C, programmable in 1° C increments
• Maximum Sample Capacity: 1536 in four 384-well plates
• Number of Sample Injections: 1 to 99 injections per sample
• Sample Delivery Precision: <0.3% RSD, full loop 50 µL. (Default wash/purge conditions, degassed 60% methanol: 40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm)
• Sample Volume Linearity: >0.999% correlation, 5 to 20 µL, partial fill in 50-µL loop, 3-µL pre- and post-sample air gaps (Default wash/purge conditions, degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm)
• Needle Wash Solvents: Two: wash (strong solvent) and purge (sample- compatible) solvent A
• Sample Carryover: <0.01% or <2.5 nL (default wash/purge volumes), whichever is greater. (Blank injection, 25 µL, 60% methanol:40% water, after six replicates, 25 µL propylparaben, 1 g/L, 257 nm)

Instrument Control Specifications

• Parallel Sample Processing: User-programmable next-sample aspiration and loop fill, column equilibration, just-in-time gradient delivery
• Advanced Operations: Auto addition, sample pooling, open access, priority samples
• Column Heater: 20° C (5° C above ambient) to 60° C, in 1° C increments

486 Specifications:

Operational Specifications

• Wavelength range (single, fixed wavelength): 190 to 600 nm
• Wavelength accuracy: ± 2 nm
• Wavelength repeatability: ± 0.25 nm
• Noise: ± 1.0 x 10-5 AU, 1 second filter setting, 210 to 280 nm) dry cell
• Drift: - 1.0 x 10-4 AU per °C (after warm-up) - 1.0 x 10-4 AU per hour (after warm-up)
• Sensitivity Setting Range: 0.001 to 2.0 AUFS (under software control) Filter Setting
• Range: 0.1 to 5.0 seconds (under software control) Bandwidth: 8 nm

Optical Component Specifications

• Lamp Source: Deuterium arc lamp
• Flow cell: Dual beam Taper-Cell design
• Path length: 10 mm (standard, analytical)
• Cell volume: 8 ul (standard, analytical)
• Pressure limit: 1000 psi
• Materials: 316 stainless steel, Dynasil Suprasil II, Teflon

Environmental Specifications

• Operating temperature: 15 to 32.2 °C
• Operating humidity: 20 to 80%, non-condensing
• Shipping and storage temperature: -40° to 65 °C
• Shipping and storage humidity: 20 to 80% non-condensing