Overview on ongoing Evaluation of Melting Curve Analysis (MCA) by EuroGentest

Wittwer, C. T. and Kusukawa, N. (2005) Nucleic acid techniques. In: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics , 4th edition (Eds, Burtis, C., Ashwood, E. and Bruns, D.), pp. 1407-1449. Elsevier, New York.

Wittwer, C.T. and Kusukawa, N (2005) Molecular Diagnostics Technology. In: Clinical Diagnostic Technology, The Total Testing Process Vol 2: The Analytical Phase (Eds, Ward-Cook, K.M., Lehmann, C.A., Schoeff, L.E., and Williams, R.H.), pp. 341 – 369. AACC Press, Washington, DC.

Highsmith WE Jr. SNPs for sale. Cheap ! Clin Chem. 2004 Aug;50(8):1296-8.

Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT. Fifty Years of Molecular (DNA/RNA) Diagnostics. Clin Chem. 2005 Mar;51(3):661-71.

Benjamin D. Horne, John F. Carlquist, Lisa A. Cannon-Albright, Joseph B. Muhlestein, Jason T. McKinney, Matthew J. Kolek, Jessica L. Clarke, Jeffrey L. Anderson, and Nicola J. Camp (2006) High-Resolution Characterization of Linkage Disequilibrium Structure and Selection of Tagging Single Nucleotide Polymorphisms: Application to the Cholesteryl Ester Transfer Protein Gene , Annals of Human Genetics, 70,524–534

C. Gregory Elliott, MD; Eric W. Glissmeyer, BS; Gregory T. Havlena, BS; John Carlquist, PhD; Jason T. McKinney, MS; Stuart Rich, MD; Michael D. McGoon, MD; Mary Beth Scholand, MD; Miryoung Kim, BS; Robert L. Jensen, PhD; Jon W. Schmidt; Kenneth Ward, MD (2006) Relationship of BMPR2 Mutations to Vasoreactivity in Pulmonary Arterial Hypertension , http://circ.ahajournals.org/, DOI: 10.1161/CIRCULATIONAHA.105.601930

Chou, L.-S., Lyon, E. and Wittwer, C. T. (2005) A comparison of high-resolution melting analysis to denaturing high performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model. Am J Clin Pathol, in press .

Willmore-Payne, C., Holden, J. A., Tripp, S. and Layfield, L. J. (2005) Human malignant melanoma: Detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis. Hum Pathol, 36: 486-93.

McKinney JT, Longo N, Hahn SH, Matern D, Rinaldo P, Strauss AW, Dobrowolski SF. Rapid, comprehensive screening of the human medium chain acyl-CoA dehydrogenase gene. Mol Genet Metab. 2004 Jun;82(2):112-20.

Willmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ. Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis. Am J Clin Pathol. 2004 Aug;122(2):206-16.

Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer CT. High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigens. 2004 Aug;64(2):156-64.

Vaughn CP, Elenitoba-Johnson KS. High-resolution melting analysis for detection of internal tandem duplications. J Mol Diagn. 2004 Aug;6(3):211-6.

Odell ID, Cloud JL Seipp M, and Wittwer CT. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting of hsp65 PCR products . Am J Clin Path 2005;123:96-101.

Dobrowolski SF, McKinney JT, Amat di San Filippo C, Giak Sim K, Wilcken B, Longo N. Validation of dye-binding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene . Hum Mutat. 2005 Mar;25(3):306-13

2006 August; Somatic Mutation Detection in Tumor Samples by Hi-Res Melting? using the LightScanner? Instrument. John Monahan, David Ruddy, Bella Gorbatcheva, Jeremy Decker, Gwendolyn Yarbrough, WeiHua Liu, Lindsey Cutler, Mikeal Wall3, Jason McKinney, Steven Dobrowolski, David H.-F. Teng

2006 July; The LightSscanner? System: Ultra Fast Mutation Discovery and Gene Scanning. R. Jones, D. de Silva, J. McKinney, S. Dobrowolski, J. Harbour, C. Ellingson, L. Nay, C. Cook, P. Riley, R. Abbott

2005 May; Analysis of the ATP7B Gene as a 2nd Tier in Prospective Identification of Wilson Disease. Steven F. Dobrowolski, Jason T. McKinney, Clinton R. Ellingson, Jesse Harbour, Jennifer Winters, Sihoun Hahn

2005 May; Analysis of the Galactose-1-Phosphate Uridyltransferase Gene in Specimens Identified through Newborn Screening for Galactosemia Using the Original Dried Blood Specimen and Hi-Res Melting? . Steven F. Dobrowolski, Jason T. McKinney, Edwin W. Naylor, Zhili Lin, Kasinathan Muralidharan, Carol J. Saunders, Fay Keune, Jesse Harbour, James B. Gibson, Clinton R. Ellingson

2005 May; Comprehensive Mutation Scanning in the Ornathine Transcarbamylase Gene using Hi-Res Melting? with LCGreen? Plus. J. T. McKinney, J. A. Harbour, C.R.Ellingson, L. Caldovic, M. Lynch, M. Tuchman, S. F. Dobrowolski

2004 May; High Resolution Melting Analysis-Based Detection of Internal Tandem Duplications of the FLT3 Gene . Cecily P. Vaughn, ARUP Institute for Clinical and Experimental Pathology and Kojo S.J. Elenitoba-Johnson, Dept. of Pathology, University of Utah School of Medicine

2004 Mar; Identification of GALT Mutations Using Specific Assays and Gene Scanning . Jason Mckinney, Matt Campbell, Steve Dobrowolski, Weiwei Zhao, Carol Saunders

2004 Mar; Validation of a Comprehensive MCAD Scanning Assay. Jason McKinney, Steve Dobrowolski, Jennifer Winters, Sihoun Hahn

Association of Molecular Pathology Conference Poster, 2003 Nov; High-Resolution Mutation Scanning of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene. Lan-Szu Chou: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah and Elaine Lyon: Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah

Association of Molecular Pathology Conference Poster, 2003 Nov; Determination of CYP2C9 Genotype by High Resolution Amplicon Melting Curve Analysis Using LCGreen I. Charles E. Hill, Alexander Duncan, and Frederick S. Nolte. Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA.

Association of Molecular Pathology Conference Poster, 2003 Nov; Detection of Exon 10 And 11 RET Mutations Using The HR-1 High Resolution Melter. Tad Holtegaard and W. Edward Highsmith, Mayo Clinic, Rochester, MN.

2003 Aug; Prospective Screening for Wilson Disease, Direct Analysis and Exon Scanning for Mutations in ATP7B. Crystal Bennett, Sihoun Hahn, Brian Dawson and Steven Dobrowolski

2003 Aug; Mutation Screening in Primary Carnitine Deficiency Patients. Dobrowolski S, McKinney J, Amat di San Filippo C, Longo N.

2003 Aug; Comprehensive, Homogenous Analysis of the MCAD Gene . McKinney J. Dobrowolski S, Longo N, Rinaldo P, Hahn S.

2003, Mar; Heteroduplex Detection with LC Green and HR-1 High Resolution Thermal Denaturation Instrument. Jason T. McKinney, Steven F. Dobrowolski, Carl T. Wittwer